Next Generation Flow™ solution

for Chronic Lymphoproliferative Disorders

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1. Stain

Pacific Blue™ OC515™ FITC PE PerCP-Cyanine5.5 PE-Cyanine7 APC APC-C750
CD20+CD4 CD45 CD8+SmIgLambda CD56+SmIgKappa CD5 CD19+TCRgd SmCD3 CD38

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Pacific Blue™ OC515™ FITC PE PerCP-Cyanine5.5 PE-Cyanine7 APC APC-C750
CD20 CD45 CD8+SmIgLambda CD56+SmIgKappa CD4 CD19 SmCD3+CD14 CD38

Go to product specifications

Characterization panels comprise the use of core markers to consistently identify the population of study in all tubes and other markers for characterization and classification of the cells in a specific disease entity.

  • The use of premixed combinations reduces operational mistakes, pipetting time and inventory management requirements. Just add the required single antibodies to complete the panel.

The design and development of the standardized 8-color antibody panels to classify each B-CLPD, T-CLPD and NK-CLPD entity, comprised the selection of both core markers and tube-specific characterization markers for the identification and characterization of all B-, T-, and NK-cell population(s) of interest (for more information check out our complete antibody catalog).

Core combination for B-CLPD panel

This lyophilized combination of core markers is suitable to identify the population of study in all tubes of the B-CLPD panel.

Pacific Blue™ OC515™ PE-Cyanine7
CD20 CD45 CD19

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2. Acquire

Omnicyt™ cytometer’s unique and innovative features make it the perfect complement to work with these panels and the complete solution for chronic lymphoproliferative disorders analysis proposed by EuroFlow™ and Cytognos:

  • Standard optical configuration with 3 lasers and 13 parameters allows to work with up to 11 fluorescences.
  • Flat top lasers guarantee the most reproducible and accurate fluorescence measurement possible and, at the same time, reduces the frequency of system calibration.
  • The non-pressurized volumetric system allows to count cells for every population in a single platform and without additional products required, as beads or cell counter.
  • Files fully compatible with EuroFlow™ Databases for LST. Electronic system capable to save up to 20 million events including all parameters (important for MRD cases).
  • No abort rate: all events are saved including aggregates which could contain pathological cells (important to follow up MRD cases).

Learn more about Omnicyt™

3. Analyze

Infinicyt™ is the most advanced software in flow cytometry data analysis containing innovative tools for multivariate analysis, integration and interpretation of multiparametric data. Infinicyt™ includes tools to access the EuroFlow™ Databases containing normal samples processed following the EuroFlow™ SOPs and stained with the LST antibody combination.

Automated Analysis:

  • The automated identification of all normal counterparts and detection of abnormal cells orientates efficiently towards the most appropriate characterization panel, reducing time and making the process user-independent.
  • EuroFlow™ Database includes peripheral blood samples from different centers reflecting biological and technical variability.

Automatic Report:

  • Automatic comparison of the frequency of each population with reference ranges from the EuroFlow™ Database.
  • Clinically relevant comments and conclusions based on reference values and patient specific-results.
  • Improves communication between clinicians and flow cytometry laboratories.
  • Available in different languages.
  • Results can be linked to your Laboratory Information System (LIS).

Learn more about the EuroFlow™ Databases

A strong technical support team with the scientific-based knowledge and practical experience to implement Next Generation Flow™

Cytognos Technical Support team have the required experience, know-how and training resources to achieve successful implementation of the NGF methodology independently of the site (public and private hospitals, research facilities or other). The following points are addressed during technical support:

  • SOPs for cytometer set-up: Instrument standardization introduced in all labs.
  • SOPs and stable lyophilized kits for sample processing: Inter and intra laboratory reproducibility.
  • Data analysis: Reference databases with standardized reports bring a common language to the different sites.

Technical support is available through email, webinars or onsite visits. Cytognos provides a variety of solutions and products specifically aimed at the establishment of Next Generation Flow™ in your lab. Feel free to contact us to know more about them.

References

  1. Costa ES, et al. A new automated flow cytometry data analysis approach for the diagnostic screening of neoplastic B cell disorders in peripheral blood samples with absolute lymphocytosis. Leukemia. 2006 May; 20:1221-1230. Go to publication.
  2. van Dongen JJ, et al. EuroFlow antibody panels for standardized n-dimensional flow cytometric immunophenotyping of normal, reactive and malignant leukocytes. Leukemia. 2012 Sep; 26(9):1908-75. Go to publication.
  3. Costa ES, et al. Automated pattern-guided principal component analysis of B-cell chronic lymphoproliferative disorders. 2010 Nov. Leukemia; 24(11): 1927-33. Go to publication.