Next Generation Flow™ solution

for Plasma Cell Dyscrasias

Download EuroFlow Workflow Poster

1. Stain

Plasma Cell Screening Tube (PCST)

  • An initial screening step based on a limited number of antibodies orientates efficiently to the need of further characterization of plasma cell dyscrasias (PCD) samples.
  • Prognostic stratification based on the expression of the β2-microglobulin antigen.
  • Suitable for evaluation of bone marrow samples.
Pacific Blue™ OC515™ FITC PE PerCP-Cyanine5.5 PE-Cyanine7 APC APC-C750™
CD45 CD138 CD38 CD56 B2-microglobulin CD19 CyIgKappa CyIgLambda

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Characterization of Plasma Cell Dyscrasias

Characterization panels comprise the use of core markers to consistently identify the population of study in all tubes and other markers for characterization and classification of the cells in a specific disease entity.

  • The use of premixed combinations reduces operational mistakes, pipetting time and inventory management requirements. Just add the required single antibodies to complete the panel.

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2. Acquire

Omnicyt™ cytometer’s unique and innovative features make it the perfect complement to work with these panels and the complete solution for plasma cell dyscrasias proposed by EuroFlow™ and Cytognos:

  • Standard optical configuration with 3 lasers and 13 parameters allows to work with up to 11 fluorescences.
  • Flat top lasers and acoustic focusing guarantee the most reproducible and accurate fluorescence measurement and, at the same time, reduces the frequency of system calibration.
  • Non-pressurized volumetric system that allows to count cells for every population.
  • Electronic system capable to save up to 20 million events including all parameters.
  • No abort rate: all events are saved including aggregates which could contain pathological cells.
  • Files fully compatible with EuroFlow™ Databases.

Learn more about Omnicyt™

* Only available in selected countries in Europe. Ask here for more details.

3. Analyze

Automated Analysis:

  • EuroFlow™ Database containing normal bone marrow samples.
  • The Reference Database includes samples collected from different centers reflecting biological and technical variability.
  • Automated identification of all normal counterparts and detection of abnormal cells reduces analysis time and makes the process user-independent.
  • Complete immune profile information is relevant for prognosis and an indicator of patients with sustained disease (e.g. high normal plasma cell recovery and an immune profile with increased B-cell maturation are indicators of good prognosis).

Automatic Report:

  • Automatic comparison of the frequency of each population with reference ranges from the EuroFlow™ Database.
  • Clinically relevant comments and conclusions based on reference values and patient specific-results.
  • Improves communication between clinicians and flow cytometry laboratories.
  • Available in different languages.
  • Results can be linked to your Laboratory Information System (LIS).

Learn more about the EuroFlow™ Databases

A strong technical support team with the scientific-based knowledge and practical experience to implement Next Generation Flow™

Cytognos Technical Support team have the required experience, know-how and training resources to achieve successful implementation of the NGF methodology independently of the site (public and private hospitals, research facilities or other). The following points are addressed during technical support:

  • SOPs for cytometer set-up: Instrument standardization introduced in all labs.
  • SOPs and stable lyophilized kits for sample processing: Inter and intra laboratory reproducibility.
  • Data analysis: Reference databases with standardized reports bring a common language to the different sites.

Technical support is available through email, webinars or onsite visits. Cytognos provides a variety of solutions and products specifically aimed at the establishment of Next Generation Flow™ in your lab. Feel free to contact us to know more about them.

References

  1. Paiva B, et al. Utility of flow cytometry immunophenotyping in multiple myeloma and other clonal plasma cell-related disorders. Cytometry B Clin Cytom. 2010 Jul;78(4):239-52. Go to publication.
  2. van Dongen JJ, et al. EuroFlow antibody panels for standardized n-dimensional flow cytometric immunophenotyping of normal, reactive and malignant leukocytes. Leukemia. 2012;26(9):1908-75. Go to publication.
  3. Flores-Montero J, et al. Next Generation Flow for highly sensitive and standardized detection of minimal residual disease in multiple myeloma. Leukemia. 2017;31(10):2094–103. Go to publication.
  4. Flores-Montero J, et al. Immunophenotype of normal vs. myeloma plasma cells: Toward antibody panel specifications for MRD detection in multiple myeloma. Cytometry B Clin Cytom. 2016 Jan;90(1):61-72. Go to publication.
  5. Rajkumar SV, et al. International Myeloma Working Group updated criteria for the diagnosis of multiple myeloma. The Lancet Oncology. 2016 Aug;17(8):e328-e346. Go to publication.
  6. Sanoja-Flores L, et al. Next generation flow for minimally-invasive blood characterization of MGUS and multiple myeloma at diagnosis based on Circulating Tumor Plasma Cells (CTPC). Blood Cancer J. 2018;8(12):117. Go to publication.
  7. Sanoja-Flores L, et al. Blood monitoring of circulating tumor plasma cells by next generation flow in multiple myeloma after therapy. Blood. 2019 Oct; 8(12):epub. Go to publication.