BulkLysis™ is a fixative-free erithrocyte lysing solution recommended for the osmotic lysis of the erithrocytes before immunofluorescence staining of peripheral blood or bone marrow aspirate samples.
BulkLysis™ protocol allows greater sensitivity in flow cytometry studies where a higher cell count is required.
This protocol is used for increasing the sensitivity of minimal residual disease detection (up to 1 cell in 100 000 cells = 0,001%) or when nucleated cell concentration needs to be increased, such as for AML/MDS panels.
BulkLysis™ is easy to apply in samples with low cell counts or to detect small/rare populations.
You can find the EuroFlow™ Standard Operating Procedure here.