The Rainbow calibration particles, 8 peaks are designed for routine calibration of flow cytometers. Rainbow calibration particles, 8 peaks are commonly used in many laboratories for Quality Control and long-term performance of the analyser.

Rainbow calibration particles, 8 peaks contain several similar sized particles that are dyed in eight different fluorescent intensities. Each particle contains a mixture of fluorophores that are excited at any wavelength from 365 to 650 nm. The same set of particles enables the calibration of all channels in the flow cytometer. The Rainbow Particles have emission spectra compatible with many common fluorochromes used for immunofluorescent staining.

Rainbow calibration particles, 8 peaks (Rainbow calibration, 8 peaks Cat. No. RCP-30-5A) are used in EuroFlow™ standardization guidelines throughout the study of initial PMT characterization, to set target MFI values and daily performance tracking of the flow cytometers.

Batch EAB01, EAC01, EAD05, EAE01, EAF01, EAG01, EAK01, EAL01.

Lower MFI (-15%) MFI 5th peak Upper MFI (+15%)
BL1 FITC 13632 16038 18443
BL2 PE 13908 16362 18817
BL3 PerCP-Cy™5.5 44601 52472 60343
BL4 PE-Cy™7 3367 3961 4555
RL1 APC 404886 476337 547787
RL3 APC-H7/APC-C750 60704 71416 82128
VL1* Pacific Blue™ 55647 65467 75287
BV™421 34695 40818 46941
VL2* Pacific Orange™ 54591 64225 73859
OC515™ 52372 61615 70857
BV™510 47987 56455 64923

* Reference values for the violet laser fluorescent channels must be selected depending on the fluorochrome used on each panel.

Omnicyt™ and OC515™ are trademarks of Cytognos Company.
Brilliant Violet™ is a trademark of Sirigen Group Ltd.
Pacific Blue™ and Pacific Orange™ are trademarks of Life Technologies Corporation.
Cy™ is a trademark of GE Healthcare.

Batch EAB01, EAC01, EAD05, EAE01, EAF01, EAG01, EAK01, EAL01.

Lower MFI (-15%) MFI 7th peak Upper MFI (+15%)
FITC 28752 33826 38900
PE 32381 38095 43809
PerCP-Cy™5.5 66846 78642 90438
PE-Cy™7 8316 9783 11250
APC 158639 186634 214629
APC-H7 64194 75522 86850
Pacific Blue™ 100542 118178 135905
Pacific Orange™ 93871 110436 127002

Batch EAB01, EAC01, EAD05, EAE01, EAF01, EAG01, EAK01, EAL01.

Lower MFI (-15%) MFI 7th peak Upper MFI (+15%)
FL1 – FITC
117719 138493 159267
FL2 – PE
101813 119780 137747
FL4- PerCP-Cy™5.5
253075 297735 342395
FL5 – PE-Cy™7
22648 26645 30642
FL6 – APC
637655 750182 862709
FL8 – APC-750
162326 190972 219618
FL9 – Pacific Blue™
379874 446911 513948
FL10 – OC515™
278720 327906 377092

EuroFlow™ is a trademark of European Scientific foundation for Laboratory Hemato Oncology (ESLHO).
FACSCanto™ is a trademark of Becton, Dickinson and Company. Cy™ is a trademark of GE Healthcare.
Navios™ and Gallios™ are trademarks of Beckman Coulter Inc.
Pacific Blue™ and Pacific Orange™ are trademarks of Life Technologies Corporation.
OC515™ is a trademark of Cytognos Company.