The Rainbow calibration particles, 8 peaks are designed for routine calibration of flow cytometers. Rainbow calibration particles, 8 peaks are commonly used in many laboratories for Quality Control and long-term performance of the analyser.

Rainbow calibration particles, 8 peaks contain several similar sized particles that are dyed in eight different fluorescent intensities. Each particle contains a mixture of fluorophores that are excited at any wavelength from 365 to 650 nm. The same set of particles enables the calibration of all channels in the flow cytometer. The Rainbow Particles have emission spectra compatible with many common fluorochromes used for immunofluorescent staining.

Rainbow calibration particles, 8 peaks (Rainbow calibration, 8 peaks Cat. No. RCP-30-5A) are used in EuroFlow™ standardization guidelines throughout the study of initial PMT characterization, to set target MFI values and daily performance tracking of the flow cytometers.

Batch EAM01.

*Select the reference target values required for each panel.

Lower MFI (-15%) MFI 5th peak Upper MFI (+15%)
BL1 FITC 10056 11831 13606
BL2 PE 12980 15271 17562
BL3 PerCP-Cy™5.5 32417 38138 43859
BL4 PE-Cy™7 2266 2666 3066
RL1 APC 76718 90256 103794
RL3 APC-H7/APC-C750™ 11021 12966 14911
VL1 Pacific Blue™ 48540 57106 65672
BV421 30192 35520 40848
VL2 Pacific Orange™ 40550 47706 54862
OC515™ 39228 46150 53073
BV510 35866 42195 48524

 

Batch EAB01, EAC01, EAD05, EAE01, EAF01, EAG01, EAK01, EAL01.

Lower MFI (-15%) MFI 5th peak Upper MFI (+15%)
BL1 FITC 13632 16038 18443
BL2 PE 13908 16362 18817
BL3 PerCP-Cy™5.5 44601 52472 60343
BL4 PE-Cy™7 3367 3961 4555
RL1 APC 404886 476337 547787
RL3 APC-H7/APC-C750™ 60704 71416 82128
VL1 Pacific Blue™ 55647 65467 75287
BV421 34695 40818 46941
VL2 Pacific Orange™ 54591 64225 73859
OC515™ 52372 61615 70857
BV510 47987 56455 64923

Batch EAM01.

Lower MFI (-15%) MFI 7th peak Upper MFI (+15%)
FITC 23292 27402 31513
PE 32666 38430 44195
PerCP-Cy™5.5 57874 68087 78301
PE-Cy™7 6774 7969 9165
APC 95180 111976 128773
APC-H7 28594 33641 38687
Pacific Blue™ 90855 106888 122922
Pacific Orange™ 72500 85294 98088

 

Batch EAB01, EAC01, EAD05, EAE01, EAF01, EAG01, EAK01, EAL01.

Lower MFI (-15%) MFI 7th peak Upper MFI (+15%)
FITC 28752 33826 38900
PE 32381 38095 43809
PerCP-Cy™5.5 66846 78642 90438
PE-Cy™7 8316 9783 11250
APC 158639 186634 214629
APC-H7 64194 75522 86850
Pacific Blue™ 100452 118178 135905
Pacific Orange™ 93871 110436 127002

Batch EAM01.

Lower MFI (-15%) MFI 7th peak Upper MFI (+15%)
FL1 – FITC 95614 112487 129360
FL2 – PE 101236 119101 136966
FL4- PerCP-Cy™5.5 218387 256925 295464
FL5 – PE-Cy™7 18706 22007 25308
FL6 – APC 366048 430645 495241
FL8 – APC-C750™ 73838 86868 99899
FL9 – Pacific Blue™ 352827 415090 477354
FL10 – OC515™ 226091 265989 305887

 

Batch EAB01, EAC01, EAD05, EAE01, EAF01, EAG01, EAK01, EAL01.

Lower MFI (-15%) MFI 7th peak Upper MFI (+15%)
FL1 – FITC 117719 138493 159267
FL2 – PE 101813 119780 137747
FL4- PerCP-Cy™5.5 253075 297735 342395
FL5 – PE-Cy™7 22648 26645 30642
FL6 – APC 637655 750182 862709
FL8 – APC-C750™ 162326 190972 219618
FL9 – Pacific Blue™ 379874 446911 513948
FL10 – OC515™ 278720 327906 377092

 

Note: EuroFlow™ target values for batch EAM01 will be published soon. For more information contact support@cytognos.com.

 

Omnicyt™, APC-C750™ and OC515™ are trademarks of Cytognos Company.
Pacific Blue™ and Pacific Orange™ are trademarks of Life Technologies Corporation.
Cy™ is a trademark of GE Healthcare.
EuroFlow™ is a trademark of European Scientific foundation for Laboratory Hemato Oncology (ESLHO).
FACSCanto™ and LSR Fortessa™ are trademarks of Becton, Dickinson and Company. Cy™ is a trademark of GE Healthcare.
Navios™ is a trademark of Beckman Coulter Inc.