EuroFlow™ Databases for Acute leukemia

Some cases of acute leukemia are not easy to diagnose, doubts arise due to the complexity of the disease, the variability of the immunophenotypic pattern, the variability in marker expression, and overlapping immunophenotypes.

EuroFlow™ developed and validated a database of representative cases, stained with the ALOT combination of antibodies following standardized operating procedures. The ALOT database, available through Infinicyt™ 2.0, is associated with algorithms implemented in a specific tool integrated in Infinicyt™ (Compass tool) specially designed to aid the users in the task of orientating for the most suitable classification panel.

Schematic overview of the construction of the EuroFlow database. Automated database-guided expert-supervised orientation for immunophenotypic diagnosis and classification of acute leukemia. Van der Velden. Leukemia 2018.

How to compare a new case with EuroFlow™ ALOT Database using Infinicyt™ Compass Tool?

  1. Identification
    The population suspected to be abnormal has to be unequivocally identified.
  2. Clustering
    The abnormal population is automatically compared with all groups of the database using all the markers in the multidimensional space.
  3. Interpretation
    The result of the processs is a Compass diagram indicating the group of the database which the population of study is more similar to (percentage of single lineage (B, T, myeloid), mixed phenotype, transitional, undifferentiated and/or immature cells.

How to Interpret the results?

The following images represent different possible outcomes:

A. Almost all events are similar to the cases from one group in the database.

B. The selected events belong to two distinct lineages; part of the events are similar to the cases from one group in the database and part are similar to the cases from a second group in the database.

C. Most of the events have a mixed phenotype between the groups indicated.

D. Some of the events are similar to the cases from one group in the database and the rest have mixed phenotype/transitional potential.

Resources

Publications:

  1. Lhermitte L, et al. Automated database-guided expert-supervised orientation for immunophenotypic diagnosis and classification of acute leukemia. Leukemia. 2017 Apr; 2(4): 874-81. Go to publication.
  2. van Dongen JJ, et al. EuroFlow antibody panels for standardized n-dimensional flow cytometric immunophenotyping of normal, reactive and malignant leukocytes. Leukemia. 2012 Sep; 26(9):1908-75. Go to publication.