Monocyte subsets tube – TiMaScan™

Evaluation

Last update: March 20th, 2020

TiMaScan™ test is rapid, accurate, broadly available and cost-effective. It has a broad applicability in several medical fields, whenever tissue damage needs monitoring.

Immunophenotype of circulating monocytes/macrophages

Identification of monocytes/macrophages by flow cytometry provides relevant information regarding homeostatic changes, such as in the case of tissue damage. The sample used for this methodology, peripheral blood, is not invasive and allows for a more adequate control of patient’s evolution and recovery.

The flow cytometry evaluation of circulating monocytes/macrophages is independent  of the time point  because the markers have been defined in consensus to be the most informative and relevant for scanning:

  • HLA-DR is expressed by hematopoietic cells at different levels and it is highly expressed on monocytes/macrophages.
  • CD45 is expressed by hematopoietic cells at different levels and it is highly expressed on monocytes/macrophages.
  • CD14 is expressed by circulating monocytes/macrophages at different levels. It is highly expressed by classic and intermediate monocytes. Non-classic monocytes show negative or very reduced expression.
  • CD300e is expressed by hematopoietic cells at different levels and it is highly expressed on mature monocytes/macrophages.
  • CD16 is expressed by circulating monocytes/macrophages at different levels. It is negative on classic monocytes, reduced to positive expression is found on intermediate monocytes/macrophages and is highly expressed by non-classic monocytes/macrophages.

Next Generation Flow™

In recent years, a solution called Next Generation Flow™ has been developed in order to create and standardize fast, accurate and sensitive flow cytometry tests. Based on that knowledge, Cytognos offers a complete and scientific-based solution to cover all the needs for Monocyte subsets tube (TiMaScan™).

Following NGF methodology, Cytognos offers the TiMaScan™ kit for rapid evaluation of circulating monocytes/macrophages (from classic to non-classic).

In order to analyze the complex amount of flow cytometry data, Cytognos offers the software Infinicyt™ 2.0 to standardize analysis.

For sample acquisition, Cytognos provides the CE-IVD Omnicyt™ cytometer, which features are compatible with NGF and make it the perfect complement to our solution for monitoring circulating monocytes/macrophages.

Resources

TimaScan™ project – Home

TimaScan™ project – Goals

TimaScan™ project – Achievements

Publications:

  1. van der Bossche WBL, et al. Flow cytometric assessment of leukocyte kinetics for the monitoring of tissue damage. Clinical Immunology. 2018 Dec; 197:224-30. Go to publication.
  2. Damasceno D, et al. Distribution of subsets of blood monocytic cells throughout life. 2019 Jul; 144(1):320-3.e6. Go to publication.
  3. Kapellos TS, et al. Human Monocyte Subsets and Phenotypes in Major Chronic Inflammatory Diseases. Frontiers in Immunology. 2019 Aug. 10:2035. Go to publication
  4. Talati, T, et all. Monocyte subset analysis accurately distinguishes CMML from MDS and is associated with a favorable MDS prognosis. Blood. 2017 Mar. 129(13): 1881-3. Go to publication
  5. Sampath P, et al. Monocyte Subsets: Phenotypes and Function in Tuberculosis Infection. Frontiers in Immunology. 2018 Jul. 9:1726. Go to publication