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SPHERO™ Rainbow calibration particles

The Rainbow calibration particles, 8 peaks are designed for routine calibration of flow cytometers. Rainbow calibration particles, 8 peaks are commonly used in many laboratories for Quality Control and long-term performance of the analyser.


Rainbow calibration particles, 8 peaks contain several similar sized particles that are dyed in eight different fluorescent intensities.  Each particle contains a mixture of fluorophores that are excited at any wavelength from 365 to 650 nm. The same set of particles enables the calibration of all channels in the flow cytometer. The Rainbow Particles have emission spectra compatible with many common fluorochromes used for immunofluorescent staining.


Rainbow calibration particles, 8 peaks (Rainbow calibration, 8 peaks Cat. No. RCP-30-5A) are used in EuroFlow™ standardization guidelines throughout the study of initial PMT characterization, to set target MFI values and daily performance tracking of the flow cytometers.


  • EuroFlow™ reference target values (7th peak) for FACSCanto™ II

        Batch EAB01, EAC01, EAD05, EAE01, EAF01, EAG01 (check values)

Lower MFI (-15%)

Rainbow 7-peak (DiVa)

Upper MFI (+15%)

Pacific Blue™

100.452

118.178

135.905

Pacific Orange

93.871

110.436

127.002

FITC

28.752

33.826

38.900

PE

32.381

38.095

43.809

PerCP

66.846

78.642

90.438

PC7

8.316

9.783

11.250

APC

158.639

186.634

214.629

APC-H7

64.194

75.522

86.850

FACSCanto™ is a trademark of Becton, Dickinson and Company. Pacific Blue™ and Pacific Orange™ are trademarks of Life Technologies Corporation. Cy™ is a trademark of GE Healthcare.
EuroFlow™ is a trademark of European Scientific foundation for Laboratory Hemato Oncology (ESLHO).


  • EuroFlow™ reference target values (7th peak) for Navios™ or Gallios™

        Batch EAB01, EAC01, EAD05, EAE01, EAF01; EAG01 (check values)

Lower MFI (-15%)

Rainbow 7-peak (Navios)

Upper MFI (+15%)

FL9 – Pacific Blue™

379,874

446,911

513,948

FL10 – OC515

278,720

327,906

377,092

FL1 - FITC

117,719

138,493

159,267

FL2 - PE

101,813

119,780

137,747

FL4 – PerCPCy™5.5

253,075

297,735

342,395

FL5 – PECy™7

22,648

26,645

30,642

FL6 - APC

637,655

750,182

862,709

AFL8 – APC-C750

162,326

190,972

219,618

Navios™ and Gallios™ are trademarks of Beckman Coulter Inc. Pacific Blue™ is a trademark of Life Technologies. Cy™ is a trademark of GE Healthcare.
EuroFlow™ is a trademark of European Scientific foundation for Laboratory Hemato Oncology (ESLHO).

 

Product
Format
Reference
Use
TDS
RAINBOW CALIBRATION PARTICLES, 8 PEAKS
5 ml
SPH-RCP-30-5A
RUO
PDF

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